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Image Search Results
Journal: Nutrients
Article Title: Dietary Fiber Intake Improves Osteoporosis Caused by Chronic Lead Exposure by Restoring the Gut–Bone Axis
doi: 10.3390/nu17091513
Figure Lengend Snippet: DF restored intestinal structure and Treg cells disrupted by Pb exposure. ( A ) Hematoxylin and eosin-stained sections ( n = 6–10) at a 200 µm scale, with insets showing 100 µm-magnified regions. Annotations: inflammatory infiltrates (red arrows), goblet cells (blue arrows), intestinal crypts (yellow arrows). ( B ) Studies on intestinal morphology involved the assessment of cumulative scores encompassing inflammatory infiltration, crypt damage, and goblet cell impairment. ( C , D ) The mRNA expression of the ZO-1 and Occludin gene in colon tissue ( n = 6–10). ( E – G ) The protein expression of the ZO-1 and Occludin gene in colon tissue ( n = 3). ( H – J ) Representative flow cytometry image of Treg cell subpopulations in the colon. ( K ) Percentage of Treg cell subsets in CD4 + T cells in mice colon ( n = 6–10). ( L – N ) Representative flow cytometry images of Treg cell subpopulations in the bone. ( O ) Percentage of Treg cell subsets in CD4 + T cells in mice bone ( n = 6–10). Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 (Dunnett multiple-comparison test).
Article Snippet: The primary antibodies involved included
Techniques: Staining, Expressing, Flow Cytometry, Comparison
Journal: World Journal of Stem Cells
Article Title: Transplanting embryonic stem cells onto damaged human corneal endothelium
doi: 10.4252/wjsc.v9.i8.127
Figure Lengend Snippet: Endothelial parts of three corneal sections: A-C, D-F, and G-I. A: Nuclei through a 4‘,6-diamidino-2-phenylindole (DAPI) filter; B: The same cells through a fluorescein isothiocyanate (FITC) filter [green fluorescent protein (GFP) fluorescence]; C: Expression of the tight junction protein Zona Occludens protein 1 (ZO-1) [tetramethylrhodamine (TRITC) filter]; D: Nuclei seen through a DAPI filter; E: GFP expression (using an FITC filter); F: Expression of sodium potassium ATPase (NaKATPase) (using a TRITC filter); G: Nuclei seen through a DAPI filter; H: GFP expression (FITC filter); I: Expression of the cornea-related protein paired box 6 (PAX-6).
Article Snippet: The secondary antibodies used were CK3 [mouse monoclonal antibody, AE5 (ab77869); Abcam, Cambridge, United Kingdom], a marker for corneal epithelial cells (Abcam); paired box 6 (PAX-6) [mouse monoclonal antibody, AD2.38 (ab78545), Abcam], a marker for a transcription factor important in the development of the eye; octamer-binding transcription factor 3/4 (OCT-3/4) [mouse monoclonal antibody, C-10 (sc-5279), Santa Cruz Biotechnology, Inc., Santa Cruz, CA, United States], a marker for embryonic stem cell activity; sodium potassium adenosine triphosphatase (NaKATPase) [rabbit monoclonal antibody (ab58475), Abcam], a marker for NaKATPase pump function; and
Techniques: Fluorescence, Expressing